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组织因子通道抑制因子2(TFPI2)活性蛋白

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)

PP5; Placental protein 5

  • 组织因子通道抑制因子2(TFPI2)活性蛋白 产品包装(模拟)
  • 组织因子通道抑制因子2(TFPI2)活性蛋白 产品包装(模拟)
  • 组织因子通道抑制因子2(TFPI2)活性蛋白 Gene sequencing
  • APB940Hu01.png Figure. SDS-PAGE
  • 组织因子通道抑制因子2(TFPI2)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Tissue Factor Pathway Inhibitor 2 (TFPI2) takes part in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. TFPI2 does not have any influence on thrombin. TFPI2 also can inhibit MMP activity, which can hydrolyze gelatin under certain conditions. Thus, the activity of TFPI2 can be measured by inhibit MMP-2 hydrolyze gelatin. Gelatin zymography is mainly used for the detection of the gelatinases, 2μg/mL was denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 8%gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubate with various concentrations of recombinant human TFPI2, then staining with coomassie brilliant blue G250, active MMP-2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel; if the activity of MMP-2 inhibit by TFPI2, there was none white binds on the gel. The result was shown in figure 1.
As the figure1 shown, MMP-2 can be inhibited by recombinant human TFPI2 at least 5μg/mL.

用法

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保?#21183;?#20869;,在?#23454;?#30340;条件下存储,损失?#23454;?#20110;5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APB940Hu01 组织因子通道抑制因子2(TFPI2)活性蛋白 Cell?culture;?Activity?Assays.
RPB940Hu01 组织因子通道抑制因子2(TFPI2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB940Hu01 组织因子通道抑制因子2(TFPI2)多克隆抗体 WB; IHC; ICC; IP.
MAB940Hu22 组织因子通道抑制因子2(TFPI2)单克隆抗体 WB; IHC; ICC; IP.
SEB940Hu 组织因子通道抑制因子2(TFPI2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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