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肿瘤坏死因子受体超家族成员7(TNFRSF7)活性蛋白

Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)

CD27; S152; T14; Tp55; CD27L receptor;

  • 肿瘤坏死因子受体超家族成员7(TNFRSF7)活性蛋白 产品包装(模拟)
  • 肿瘤坏死因子受体超家族成员7(TNFRSF7)活性蛋白 产品包装(模拟)
  • APB043Hu01.jpg SDS-PAGE图
  • 肿瘤坏死因子受体超家族成员7(TNFRSF7)活性蛋白 Figure. Western Blot; Sample: Recombinant TNFRSF7, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

TNFRSF7 (Tumor necrosis factor receptor superfamily member 7), also known as CD27 antigen, is a member of the TNF-receptor superfamily. This receptor is thought to be involved in tumor necrosis factor-activated receptor activity by binding with TNFs. Thus, a binding ELISA assay was conducted to detect the association of TNFRSF7 with TNFa. Briefly, recombinant human TNFRSF7 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF7 with TNFa was shown in Figure 1 and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保?#21183;?#20869;,在?#23454;?#30340;条件下存储,损失?#23454;?#20110;5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPB043Hu01 肿瘤坏死因子受体超家族成员7(TNFRSF7)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB043Hu01 肿瘤坏死因子受体超家族成员7(TNFRSF7)活性蛋白 Cell?culture;?Activity?Assays.
PAB043Hu01 肿瘤坏死因子受体超家族成员7(TNFRSF7)多克隆抗体 WB; IHC; ICC; IP.
FAB043Hu01 抗肿瘤坏死因子受体超家族成员7(TNFRSF7)多克隆抗体 Flow cytometry.
LAB043Hu71 肿瘤坏死因子受体超家族成员7(TNFRSF7)多克隆抗体(生物素标记) WB; IHC; ICC.
FAB043Hu41 抗肿瘤坏死因子受体超家族成员7(TNFRSF7)多克隆抗体(藻红素标记) Flow cytometry.
FAB043Hu51 抗肿瘤坏死因子受体超家族成员7(TNFRSF7)多克隆抗体(别藻蓝蛋白标记) Flow cytometry.
FAB043Hu81 抗肿瘤坏死因子受体超家族成员7(TNFRSF7)多克隆抗体(异硫氰酸荧光素标记) Flow cytometry.
MAB043Hu21 肿瘤坏死因子受体超家族成员7(TNFRSF7)单克隆抗体 WB; IHC; ICC; IP.
FAB043Hu02 抗肿瘤坏死因子受体超家族成员7(TNFRSF7)单克隆抗体 Flow cytometry.
FAB043Hu42 抗肿瘤坏死因子受体超家族成员7(TNFRSF7)单克隆抗体(藻红素标记) Flow cytometry.
FAB043Hu52 抗肿瘤坏死因子受体超家族成员7(TNFRSF7)单克隆抗体(别藻蓝蛋白标记) Flow cytometry.
FAB043Hu82 抗肿瘤坏死因子受体超家族成员7(TNFRSF7)单克隆抗体(异硫氰酸荧光素标记) Flow cytometry.
SEB043Hu 肿瘤坏死因子受体超家族成员7(TNFRSF7)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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